Sennheiser EM 3731-II - 09-09 Instruction Manual download pdf (Page 25)

Page 21 Leica EM CPD030 Operating Manual 09/09

5.2 Preparation of Specimen

Remove the specimen from the organ or the culture medium.

Wash in a physiological salt solution.

Fix chemically with a suitable fixing agent.

Wash out fixing agent with a suitable buffer solution.

Dehydrate the chemically fixed specimen with acetone or ethanol. If ethanol is used

as a dehydration medium, which does not mix well with the transition liquid CO2, it

must first be transformed step by step into the intermediate amylacetate or acetone.

Note!

The specimen to be critical point dried is prepared in the same way as a

sample for ultramicrotomy.

5.3 Filling the specimen pressure chamber with acetone

Open the screw-on cover of the specimen pressure chamber by turning counter

clockwise.

Before filling the specimen pressure with acetone, place the magnetic stirrer rod in

bottom of the chamber and then cover with the aperture disc, if this has not already

been done during installation.

Fill the specimen pressure chamber with acetone to such a level that the specimen

container and specimen will be completely submerged when inserted.

Screw-on cover

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Sennheiser EM 3731-II - 09-09 Instruction Manual Page 25